Chitosan/alginate complexes were prepared at different polycation/polyanion molar ratios and freeze-dried vaginal inserts were obtained for chlorhexidine digluconate local delivery in genital infections. Complex yield, FT-IR spectra, and TGA thermograms were studied to confirm the interaction between the two polyions. The influence of different complexes on physical handling, morphology, and drug distribution in the samples were evaluated by friability test, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS), respectively.
In vitro water-uptake, mucoadhesion and release tests were performed as well as microbiological tests toward pathogenic vaginal microorganisms. The results showed that the selection of suitable chitosan/alginate molar ratio and drug loading allowed modulate insert ability to hydrate, adhere to the mucosa, and release chlorhexidine digluconate. The insert containing an excess of alginate was found to be the best performing formulation and showed good antimicrobial activity toward the pathogens Candida albicans and Escherichia coli.
Study the Chlorhexidine digluconate bactericidal effect of a compound disinfectant and ethanol toxicity. Methods Refer to "disinfection technical specifications" method, suspension quantitative germicidal test and animal toxicity testing methods were studied. The results containing chlorhexidine gluconate 2000mg / L and 53% ethanol disinfectant against Staphylococcus aureus, Escherichia coli 0.5min killing logarithm value was> 5.00; Candida albicans 0.5min killing logarithm value> 4.00 rubbed his hands in the disinfectant effect. 1.0min, the average killing natural bacteria on the surface of the opponent as a value> 2.45, and the killing logarithm values ​​of each trial were> 1.00. acute oral toxicity tests in rats and mice, LD50 was> 5000mg / kg BW, genera the actual non-toxic, non-irritating to the skin of rabbits, 500 ~ 5000mg / kg Bw doses of mouse bone marrow micronucleus test results of the test group and negative control group, the difference was not significant, and no mutagenic effect; subacute toxicity test results, dose at 1000mg / kg BW or less, compared with the negative control group, the rats of the body weight, the number of dirty system, blood and serum had no significant impact on organ histopathology no abnormal Conclusion chlorhexidine gluconate complex in ethanol disinfectant low toxicity, small skin irritation, can be used for skin disinfection use. this study disinfectant alcohol content of 53%, somewhat lower than in a conventional concentration of 70%, an increase of water content, easy to reach (without washing after use), in order to avoid long-term use of rough dry skin problems.