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Permeation of chlorhexidine digluconate through hairless rat skin

          The use of Chlorhexidine digluconate on the skin, mucosa, sores and burns served as basis for the study of percutaneous penetration of a 5% antiseptic solution (HIBITANE(R) 5%). Tests were made in permeation cells (FRANZ type) for 48 h on whole hairless rat skin or hairless rat skin without stratum comeum. 2 ml of HIBITANE(R) 5% was used as donor solution and 10 ml of NaCl 0,9%, renewed for each sampling, was used as receptor solution. HPLC was used to close the quantity of Chlorhexidine digluconate in the samples taken at time 1, 3, 9, 24, and 48 hours and in the cutaneous structures after 48 hours. The results obtained have been reproduced in the table below :It can be seen from the foregoing data that the integrity of the skin and, specially, the presence of the Stratum Corneum, play a very important role in diffusion. The total quantity diffused through the stripped skin is a 100 times more than that observed for the whole skin, no true lag time was observed, no steady state flux could be computed either.Tests carried out on whole skin revealed that the storage is more important than the diffusion to the receptor. The reverse was observed for stripped skin, where the quantity stored was slightly less than the total quantity diffused after 48 h. These results suggest the Chlorhexidine digluconate fixation in the cutaneous lipids where it probably forms micelles. The high rate of percutaneous penetration through the skin without stratum corneum should therefore be taken into account when using this antiseptic solution on cutaneous lesions.

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