Chlorhexidine is an antiseptic widely used to clean the skin. The present work compare the percutaneous diffusion of Hibitane through hairless rat skin with or without stratum corneum. For the tests carried out on whole skin, the storage is more important than the diffusion; the reverse was observed for stripped skin. The results are discussed according the composition of the commercial solution and the physicochemical characteristics of chlorhexidine digluconate.